Triploidy appears to be a promising method for developing new seedless tangerine hybrids. Triploid citrus hybrids are usually sterile, though they may occasionally produce fruit with few seeds. As a result, flow cytometry was used to determine the level of ploidy of embryos from these seeds, and two culture media were tested in vitro for their germination in vitro. The embryos were cultured in vitro on a Murashige and Tucker medium (1969) according to two compositions: M1 = MT + 1 mg / l gibberellic acid and M2 = MT + 0.25 g / l adenine sulphate, extracted from the ripe fruit of the triploid mandarin. Flow cytometry was used to identify the triploid seedlings at the INRA Kenitra laboratory level. The in vitro germination rate of embryos in M1 and M2 media is 86 percent and 78 percent, respectively. According to the mandarin hybrids, it is 86 percent and 79 percent for HT11 and HT44, respectively. Furthermore, flow cytometry analysis of the leaves of young seedlings showed that the HT11 hybrid has a triploidy rate of 94 percent and the HT44 hybrid has a triploidy rate of 38 percent. The preliminary findings of this study show that sterile triploid hybrids can produce diploid or triploid seeds on their own. Similarly, the degree of sterility differs depending on the triploid hybrids' ability to produce seeds.
In a later study, molecular markers will be used to identify the genetic origins of the diploid and triploid seedlings acquired (zygotic or nucellar).Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5558
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