CONTROLLING OF Streptococcus mutans AND Lactobacillus IN In-vitro USING HERBAL FORMULATION OF Ocimum sanctum AND Justicia adhatoda MEDIATED SILVER NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Introduction: Nanomedicine has enormous potential for improving human disease diagnosis and treatment. The use of microbes in the biosynthesis of nanoparticles is a process that is environmentally friendly. Nanoparticles come in a variety of shapes and sizes, including gold, silver, alloys, and magnetic particles. The antimicrobial properties of silver nanoparticles are well known.

Tulsi leaves can be used to treat a variety of oral infections. This plant contains pyrochemical agents such as carvacrol and terpene. Justicia adathoda, also known as ‘Vasaka,' is an antibacterial, anti-inflammatory, anti-fungal, and anti-oxidant with minimal cytotoxic and anti-oxidant properties.

Aim: To use herbal formulations of Justicia adhatoda and Ocimum santum mediated Silver Nanoparticles to control the activity of Streptococcus mutans and Lactobacillus in vitro.

1. To test the antibacterial activity of biosynthesised Ag nanoparticles with Adhatoda and tulsi.

2.To facilitate the biosynthesis of Ag nanoparticles mediated by Justicia adhatoda and Ocimum santum over other artificially and chemically formed AgNps.

Materials and Methods: Dried leaf extracts of Tulasi and Justicia adhatoda were used as reducing agents in the synthesis of cost-effective and environmentally friendly silver nanoparticles, which were then tested against Streptococcus mutans and Lactobacillus. The activity of silver nanoparticles was monitored using UV-Visible spectroscopy, and the activity revealed significant inhibitory effects against Streptococcus mutans and Lactobacillus.

Conclusion and Findings: The highest concentration of the extract, 150 l, was found to have the greatest zone of inhibition against both Streptococcus mutans and Lactobacillus. As a result, the extract was discovered to have potent antimicrobial properties. Between the wavelengths of 400 nm and 450 nm, the peak was highest. In the current study, AgNPs made from a combination of the herbs Justicia adhatoda and Ocimum santum showed significant antibacterial activity due to their potent inhibitory action against bacterial colonies.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5561