The aim of this study was to find out how common antibiotic resistance is in Pseudomonas aeruginosa by using phenotypic and genotypic detection of antibiotic resistance genes using PCR techniques. From March to May 2019, a total of 72 tonsillitis clinical samples were collected from AL-Hakeem Hospital and AL-Sadder Medical City in AL-Najaf province. According to the results of this analysis, 20/55 (36.36%) of isolates were classified as Pseudomonas aeruginosa using a cultural, microscopic, biochemical, and automated compact vitek-2 device with GN-ID card. Antimicrobial susceptibility was tested on twenty isolates of Pseudomonas aeruginosa. The antimicrobial strength levels of the 20 isolates were Norfloxaxin (96.1%), Ciprofloxacin (96.1%), Tobromycin (92.3%), and Nalidixic acid (92.3%), according to the results of the current analysis (76.9 percent ). Gentemycin (30.7 percent) and Cefotaxim (30.7 percent) were found to have a moderate strength quality (30.7 percent ). Tetracycline and amoxicillin had the lowest resistance rates (3.8 percent and 11.5 percent, respectively). Out of the 14 isolates that yield positive results in Congo red agar for biofilm formation, 10/14 (71.4%) were positive for the pslG gene, according to the findings.
Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5596
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