Background: Roscoe belongs to the Zingiberacea genus, and the plant ginger (Zingiber officinale) is a perennial herb with dense and tuberous rhizomes. Plants have long been used in the preparation of traditional medicines, and those drugs were made from locally cultivated wild plants. The aim of this study was to see how effective E. elatior flower's methanol extract was against a variety of disease-causing fungi and bacteria, as well as to look into antimicrobial sources and report on antioxidant activities and phytochemical screaming tests.
Methodology: All experiments were carried out at the University of Lahore's
biotechnology and plant science laboratories. Using an ethanol extraction
system, an N-hexane extraction system, and a rotary evaporator for aqueous
extraction. n-hexane, ethanol, whitman filter paper, nutrient agar, SDA (Sabouraud
Dextrose Agar), distilled water, and nutrient Broth were all used in this
experiment or reagent analysis and were given as follows: n-hexane, ethanol,
whitman filter paper, nutrient agar, SDA (Sabouraud Dextrose Agar), distilled
water, and nutrient The extracts were held in autoclaved 25 mL McCartney
bottles in a 4°C refrigerator until they were required. Prior to storage, the
bottles were carefully labelled and labelled correctly.
The antifungal efficacy of Zingiber officinale was assessed against multiple
forms of pathogens with different concentrations such as Aspergillus flavus,
Aspergillus flavus, and Aspergillus niger, as well as the positive controls.
Disc diffusion is used to apply all forms of fungus to various fungus cultures.
First and foremost, we remove the rhizome under fungus regulation. Rhizome
extract provides the best results in zone of inhibition in Aspergillus niger
and Aspergillus flavus (17 mm and 17.5 mm, respectively), but other forms of
fungi species also provide good results.
Conclusion: The antimicrobial activity of n-hexane extract prepared from ginger
rhizome was dose dependent in this analysis (Zingiber officinale). Along with
the positive controls, there were four bacterial strains: Bacillus cereus,
Escherichia coli, Aceromonas, and Pseudomonas aeruginosa. Aceromonas,
Pseudomonas aeruginosa, and Pseudomonas aeruginosa displayed the highest
antibacterial activity, which was higher than the positive regulation, Zingiber
officinale. The minimum antibacterial ethanol extraction, on the other hand,
was discovered against Escherichia coli. Inhibition areas were not visible in
the positive regulation.
Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5583
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