One of the prerequisites for breeding activities is the estimation of genetic diversity of plant materials, as the effectiveness of breeding programs depends on the genetic variability dimension. The present study was performed using ten simple sequence repeat (SSR) loci to establish genetic divergence and molecular relationships with 32 genotypes of yardlong bean, including commercial variety and inbred lines. Among the 32 yard-long bean genotypes with an average of 3.2 alleles per locus, a total of 32 alleles were identified. In this experiment, total mean polymorphism information content (PIC) and Nei gene diversity of 0.3285 and 0.3662 were found. The highest allele frequency (0.9688) but the lowest genetic diversity and PIC was given by the SSR primer VM35. Cluster analysis based on similarity indices (UPGMA) grouped 32 genotypes into four groups, one of which was further divided into sub-clusters and sub-sub-clusters. It is important to note that three genotypes remain unclustered, namely, BD-9833, Lal Benny, and Saba. Within cluster III, the majority of genotypes were found (90.63 percent ). In a number of ways, the SSR analysis resulted in the conclusive separation of genotypes, suggesting the existence of large heterogeneity between genotypes that can be used for the potential breeding program.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5520
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