In vitro PROPAGATION OF Cryptocarya stocksii Meisn. -A VULNERABLE TREE FROM INDIA | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

By using a cotyledonary node, the current work describes an efficient, high-frequency multiple shoot induction protocol. Cryptocarya stocksii seeds were sterilised with HgCl2 (0.1 percent w/v) on the surface for 7 min and washed with sterile water 3 times. Seeds were grown on a medium containing 0.5 mg/L Thidiazuron (TDZ) for 20 days on Murashige and Skoog (MS). Cotyledonary nodes were excised as explants for multiple shoot induction from 20-day-old seedlings. Cotyledonary nodes containing 1 mg/L TDZ, 0.5 mg/L 1-Naphthaleneacetic acid (NAA), 0.5 mg/L Gibberellic acid (GA3) and 0.2 percent Activated charcoal (AC) for multiple shoot induction were pre-inoculated for 20 days on MS medium. Subsequently, MS media containing 3 mg/L 6-Benzylaminopurine (BAP), 0.5 mg/L NAA and 1 mg/L Kinetin were transferred to the secondary medium for shoot elongation (KN). The 4 to 5 leaf shoots were transferred to the root induction medium of MS containing 3 mg/L indole 3-butyric acid (IBA). Plantlets with well-developed roots were transferred and maintained in the growth room to pots containing sterile sand.

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