During the 2018/2019 growing season, this study was performed at the Plant Tissue Culture Laboratory, College of Agriculture, University of Basrah. The goal of the study was to use the segments of the capitulum as explants in the micro-propagation of the gerbera plant cultivars of Orange and Malibu. The results showed that the capitulum segments of the gerbera vine, cultivars of Orange and Malibu, cultivated in vitro as explants, resulted in the direct proliferation of adventitious shoots. The results also show the important superiority of the orange cultivar in its response rate to direct shoot regeneration, which, after 12 weeks of cultivation, reached 86.72 percent. In the number and length of the shoots that were produced from direct organogenesis, the Malibu cultivar was substantially superior, which reached 10.48 shoots explant-1 and 1.78 cm respectively. The 1.0 mg L-1 BA concentration was significantly higher than the other explant response concentrations for direct shoot proliferation and the number and duration of shoots observed were 99.22% and 15.90 shoots for explant-1 and 1.78 cm, respectively. While the explants cultivated on the MS medium supplied with 5.0 mg L-1 BA showed the lowest response to direct shoot proliferation and the number and length of shoots, which reached 27.50% and 2.00 shoots, respectively, explant-1 and 0.35 cm.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5530
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