Aim: The aim of the study is to synthesis Hybanthus enneaspermus mediated silver nanoparticles and to evaluate the cytotoxic and antioxidant potential.
Introduction: Hybanthus enneaspermus (F. Muell) belongs to the family
Violaceae. The plant Hybanthus enneaspermus is reported to have antimicrobial,
anticonvulsant, nephroprotective, hepatoprotective, anti-inflammatory,
aphrodisiac, aldose reductase inhibitory and free radical scavenging
activities. Ag NPs produce reactive oxygen species and free radicals causing
apoptosis (cell death) thereby preventing their replication. Many researchers
have correlated the plants antioxidant potential with their phenolic
constituents. Antioxidants have resistance against oxidative stress by
scavenging free radicals, thereby preventing many diseases.
Materials and
Methods: Cytotoxic activity: Brine shrimp eggs were obtained from the new aqua
laboratory. Filtered artificial seawater was prepared, then shrimp eggs were
added into the dark side of the chamber while there was a lamp above the other
side to attract the hatched shrimp. Two days were allowed for shrimp to mature,
after two days the shrimp Larva was ready. 10 brine shrimps were added
accordingly to the silver nanoparticle. It added 5, 10, 15, 20 micrometer and
it was left for 24 hrs and observation was made.
Antioxidant
activity: 50% methanol, DPPH solution and Hybanthus enneaspermus mediated
silver nanoparticles were added in 5 test tubes ranging from 10-50 microliters
and kept in a dark place for 10 minutes and the reading was recorded using
photometry.
Results and
Discussion: In the cytotoxic activity as the concentration of the silver
nanoparticle increases the percentage of lethality of brine shrimps also
increases. In the antioxidant activity as the concentrations of silver
nanoparticles increases the percentage of inhibition also increases.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5359
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