Aim: The aim of the study was to assess the antioxidant and antimicrobial activity of lycopene extract against oral pathogens.
Materials and
Methods: Lycopene extract was prepared and the antimicrobial activity was
tested against Candida albicans, Streptococcus mutans (gram +), and
Lactobacillus sp. 2,2-diphenylpicrylhydrazyl ( DPPH )radical scavenging assay
was used to test antioxidant activity. 2 ml of lycopene extract in the
concentration range of 10-50 μl was prepared, Absorbance value was measured
spectrophotometrically at 517 nm. To
measure the antioxidant activity the DPPH radical scavenging assay was used. 2
ml of lycopene extract in the concentration range of 10-50 μl was prepared in
50% methanol solution and was added to equal volume of 0.1 mM of DPPH solution.
The reaction mixture was incubated for 30 min in dark at room temperature. Absorbance
value was measured spectrophotometrically at 517 nm.
Results: From
present study we can infer that for antimicrobial activity the zone of
inhibition of lycopene at a
concentration of 50 µl was 8 for S. mutans, Lactobacillus and Candida, at concentration of 100 µl was 9,10 and 8 for
S. mutans, Lactobacillus and Candida respectively, at concentration of 150 µl
was 13,14 and 9 for S. mutans, Lactobacillus and Candida respectively. The zone
inhibition of lycopene was maximum for lactobacillus at concentration of 150
µl.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5325
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