Background: Zinc oxide nanoparticles have received much attention recently due to its properties such as its extensive antimicrobial activity, eco-friendliness, and simplicity. ZnO especially has special attention due to its excellent antibacterial nature. In addition to these properties, Zinc oxide nanoparticles were also found to be less toxic in in-vitro tests. In the current study we have chosen grape seed due to its protection against oxidative damage, and anti-cholesterol, anti-diabetic and antiplatelet properties.
Aim: The aim
of the current study was to analyse the antioxidant activity of grape seed
mediated zinc oxide nanoparticles.
Materials and
Methods: Collected grape seeds were powdered and stored in an air-sealed
container. 1g of grape seed extract was mixed with 100 ml of distilled water
and is boiled for 15 minutes. This solution is then filtered, to which 20 ml
distilled water is added. To this solution 0.862 g of zinc oxide is added and
was subjected to constant stirring untitled the formation of white suspension.
This is centrifuged for 10 minutes and the antioxidant property against DPPH
free radical scavenger is assessed.
Results: The
percentage inhibition was only 43.8% when the concentration was 5 μl, at 10 μl
it was 46.4%, at 15 μl it was 49.9%, at 20 μl it was 58.3% and at 25 μl it was
60.2%. It was observed from the spectra that the extract at 517 nm had highest
radical scavenging activity at a concentration of 25 μl (60.2%), which is
indicative of significant antioxidant activity as potent as DPPH itself.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5334
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