Friday, January 22, 2021

ANTIOXIDANT ACTIVITY OF GRAPE SEED MEDIATED TiO2 NANOPARTICLES: AN In vitro STUDY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: With the development of nanotechnology, there has been a tremendous growth in the application of nanoparticles for drug delivery systems, antibacterial materials, cosmetics, sunscreens, and electronics. Titanium dioxide (TiO2) nanoparticles (NPs) are manufactured worldwide for a variety of engineering and bioengineering applications. TiO2 NPs under ultraviolet radiation produce a strong oxidative effect through the formation of above-mentioned reactive oxygen species and can be used as a photocatalytic disinfectant without other chemical reagents.

Aim: To assess the antioxidant activity of grape seed mediated titanium dioxide nanoparticles.

Materials and Methods: The collected fruit seeds were washed dried powdered and subjected to nanoparticles synthesis by adding titanium oxide solution to the grape seed extract solution. The solution was centrifuged and checked for absorbance using the spectrometry. The antioxidant property of the extract was evaluated using the DPPH (2, 2-diphenylpicrylhydrazyl) assay. The results were analyzed and graphs were plotted.

Results: The titanium nanoparticles showed strong spectrophotometric absorbance around 370 nm. When subjected to DPPH assay to check for its antioxidant activity, grape seed mediated titanium oxide nanoparticles showed highest antioxidant activity at a concentration of 50 μl (80%).

Conclusion: The grape seed mediated TiO2 showed efficacious and potent antioxidant properties and therefore can be used for further applications in the medical field.

Please see the link :-
https://www.ikprress.org/index.php/PCBMB/article/view/5381

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