Aim: To evaluate the antimicrobial efficacy of Neem, calcium hydroxide and combination of both as an intracanal medicament against E. faecalis.
Background:
Azadirachta indica is one of the most commonly used medicinal plant in India.
Neem has many properties like antibacterial, antifungal activity against various
pathogenic microorganisms. E. faecalis is a part of normal flora of the mouth
and is an extremely resistant strain of microorganisms that is present even
after endodontic treatment causing reinfections.
Materials and
Methods: 2 g of Neem powder was diluted in 100 mL distilled water and was
boiled for 10 minutes, this solution was filtered using a blotting paper into a
beaker. The Neem extract was further reduced to 5 mL by boiling. It was then
measured on agar plates using agar diffusion method. Muller Hinton Agar media
was the selective media for the growth of E. faecalis. Agar diffusion method
was used to assess the antimicrobial efficiency. The test groups were Group A:
Neem, Group B: Calcium Hydroxide, Group C: Neem + Calcium Hydroxide. Zone of inhibition
was measured. The values were subjected to statistical analysis. The
significance level was set at 0.05.
Results: All
three groups exhibited antimicrobial property against E. faecalis. Zone of
inhibition was greater for Group B (21 mm) followed by Group C (17 mm) and
Group A (16 mm) against E. faecalis. Kruskal Wallis analysis showed significant
difference between the groups (p<0.05, p-0.00193).
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5313
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