The present study was
carried out to develop an efficient plant regeneration protocol for Sesamum
indicum (L.) from cotyledon explants prepared from 10-day-old in vitro grown
plantlets. Cotyledon explants inoculated on MS medium containing 2 mg l−1
N6 –Benzyladenine (BA) and 30 mg l−1 spermidine induced
the higher number of shoots (23.43 shoots/explant) and the induced shoots were
elongated (5.63 cm/shoots) in the same medium. The maximum frequency (92.33%)
of root induction was recorded in MS medium fortified with 10 mg l−1
putrescine. Up to 95% of the regenerated plantlets acclimatized and developed
further under the greenhouse conditions. Antibacterial activity of the
regenerated plant extracts exhibited resistance against various pathogens such
as, Escherichia coli, Vibrio cholerae, Bacillus subtilis and Micrococcus
luteus. Highest zone of inhibition (13 mm) was recorded for B. subtilis and M.
luteus at 50 μg ml−1 extract.
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