The aim of this study was to look at the morphological response and expression patterns of the PFP (Pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase), CYP81D8 (Cytochrome P450 gene), P5Cs (Pyrroline-5-carboxylate synthetase), and SOD (Superoxide dismutase) genes in sugarcane plants that were kept wet. For morphological studies and RNA isolation and gene expression analysis, waterlogged and control plants of four sugarcane varieties, CoLk 94184, BO 91, CoS 767, and CoJ 64, were used. Waterlogged plants had a higher plant height, but their leaf area and SPAD index were significantly lower than control plants. The emergence of aerial rooting earlier in the season appears to be linked to further waterlogged shoot root injuries. Due to waterlogging, degree Brix (°Brix) in cane juice was higher in most of the studied varieties except BO 91; variety CoLk 94184 showed the highest increase (66.7 percent) over control. The transcript of the PFP enzyme (phospho fructokinase), which is involved in glycolytic reactions, was up-regulated in two varieties (90.5 percent in CoLk 94184), but down-regulated in others. The CYP81D8 (ROS associated proteins) gene showed a significant increase (+110 percent in CoLk 94184) and BO 91 (13.6 percent) in waterlogged plants, but a small decrease in CoS 767 (-7.7%,) and CoJ 64. (-5 percent ). Under waterlogged conditions, P5CS, which is involved in proline biosynthesis, is slightly increased. Waterlogging caused SOD, an antioxidant enzyme, to increase significantly; the highest increase (+154 percent) was seen in the CoLk 94184 variety. The expression of these genes was found to have a close relationship with shoot and leaf characteristics.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5631
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