Background: The stimulant, antiseptic, antifungal, and antibacterial properties of betel leaf have long been established in Indian traditional medicine. It has been used as an anti-caries agent in toothpastes and mouthwashes. Streptococcus mutans is the most common bacteria involved in caries pathogenesis.
The aim of this study is to see how chewing betel leaves affects salivary pH and the viability of Streptococcus mutans.Materials and Methods: Before and after chewing betel leaves, 60 samples from 30 subjects were obtained. pH strips were used to determine the pH of the saliva. After that, saliva samples were diluted to a 1:160 ratio and streaked onto Mutans-Sanguis agar. The colonies were counted after the agar plates had been incubated at 37°C for 24 hours. Using the Statistical Package for Social Sciences (SPSS) for MacOS, the data was then subjected to a Paired Sample T-Test (Version 21, 2020).
The number of colonies produced in the sample taken before chewing betel leaves was 41.90 on average, and 25.80 after chewing. (p=0.004; p0.05; statistically significant) Although the data was statistically insignificant, the average pH of the sample before chewing betel leaves was 5.995 and that of the sample after chewing betel leaves was 6.190. (p=0.081; p>0.05; statistically insignificant)
Conclusion: The bacterial colony count has decreased, suggesting that betel leaves have antibacterial activity against Streptococcus mutans. While the pH has risen slightly, the data remains statistically insignificant.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5612
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