The study's goal was to make Amla seed-mediated silver nanoparticles, graphene oxide nanoparticles, and GO-Ag nanocomposite and test their antibacterial activity against Streptococcus mutans, Lactobacillus, and Candida albicans. The cytotoxicity of amla seed mediated graphene oxide nanoparticles and silver nanoparticles, as well as GO-Ag nanocomposite, is then determined using the Brine shrimp lethality assay.
Amla seed extract was prepared using the following materials and methods. Silver, graphene oxide nanoparticles, and GO-Ag nanocomposites were biosynthesised using the filtrate. The samples were measured for maximum absorbance using UV-Visible spectrophotometry after 24 hours of incubation. To produce the synthesised silver nanoparticles, graphene oxide nanoparticles, and GO-Ag nanocomposite, all of the samples were heat dried. Various concentrations of biosynthesized Amla seed mediated silver nanoparticles, graphene oxide nanoparticles, and GO-Ag nanocomposite (50,100,150 g/ml) were tested for antibacterial activity against Streptococcus mutans, Lactobacillus, and Candida albicans. The cytotoxicity of different nanoparticle concentrations was determined using the Brine Shrimp Lethality Assay.
The colour of AgNPs changed from colourless to reddish brown, the colour of GO NPs changed from brown to black, and the colour of GO-Ag nanocomposite changed from black to a darker intensity of black. The formation of their respective nanoparticles is confirmed by the colour change. Antibacterial activity of silver nanoparticles against Streptococcus mutans.
Lactobacillus (150 ug/ml – 17 mm zone of inhibition) and Lactobacillus (150 ug/ml – 20 mm zone of inhibition) all showed 20% lethality at a concentration of 25 ul. Graphene oxide nanoparticles have strong antibacterial activity against Lactobacillus (150 ug/ml – 20 mm zone of inhibition) and demonstrated 40% lethality at a concentration of 25 ul. The antibacterial activity of the GO-Ag nanocomposite was negligible against all of the test organisms, with 10% lethality at 20 and 25 ul. Antibacterial activity against Candida albicans was limited in all three nanoparticles (9 mm Zone of Inhibition).
Conclusion: Amla fruit seed extracts were used to make silver nanoparticles, graphene oxide nanoparticles, and GO-Ag nanocomposites. Silver, graphene oxide nanoparticles, and the GO-Ag nanocomposite all had a strong antibacterial impact against oral pathogens while causing minimal cytotoxicity.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5555
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