In vitro ANTI-INFLAMMATORY ACTIVITY OF Vitis vinifera SEED EXTRACT USING ALBUMIN DENATURATION ASSAY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Introduction: Inflammation is the immune system's primary reaction to harmful stimuli, such as bacteria, infected cells, radioactive substances, or irradiation, and works by eliminating harmful stimuli and initiating the healing process. Like flavonoids, polyphenols, anthocyanins, proanthocyanidins, procyanidins, and the stilbene derivative resveratrol, Vitis Vinifera contains multiple active components. In particular, grape seed extract has been reported to have a wide range of pharmacological and therapeutic effects, such as cardioprotective, hepatoprotective and neuroprotective effects, as well as antioxidant, anti-inflammatory and antimicrobial activities.

Objective: The purpose of the study is to assess the anti-inflammatory activity of grape seed extract using an albumin denaturation assay.

Materials and Methods: First, a concentrated grape seed extract solution was manufactured. This solution was then tested using the Albumin Denaturation Assay at various concentrations (10-50 ug/mL) for its anti inflammatory effect. At 660 nm, the absorbance value was registered.

Results: Grape seed extract has been shown to have strong anti inflammatory activity through this experiment. It has been shown to have stronger anti-inflammatory properties than the sodium-diclofenac norm. It has less side effects than the standard, too. It contains polyphenols, which are responsible for this effect in large part.

Conclusion: The absorbance rate increases at 660 nm with increasing concentrations of grape seed extract, suggesting that the percent inhibition of GSE also increases in the same way.

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