The study was carried out to determine the effect of plant growth regulators (PGRs) on four Bangladeshi tomato varieties on in vitro regeneration. For successful regeneration by callus induction, three explants (cotyledon, hypocotyl, and leaf segments) from four tomato varieties named-BT-2 (BARI Tomato-2), BH-T4 (BARI Hybrid Tomato-4), Apple Tomato, and Native Tomato were used. For callus induction and subsequent plantlet production, MS medium fortified with varying concentrations and PGRs combinations were used. Based on varieties, forms of explants, and hormonal combinations, the nature and texture of the callus were different. In MS+1.0 mg/L BAP + 1.0 mg/L NAA for leaf segment and cotyledon derived BH-T4 and Apple tomato variety explants, the finest callus induction was reported. Full shoot regeneration was achieved with 1.0 mg/L BAP+0.5 mg/L NAA and 0.5 mg/L NAA+1.0 mg/L IAA hormonal combinations with the highest root induction. Well rooted, regenerated plantlets were eventually acclimatized and 70 percent was the best survival rate after acclimatization. Among the four varieties, the most sensitive genotypes were found to be the BH-T4 and apple tomato varieties in terms of different parameters such as primary culture establishment capacity, callus induction rate, shoot initiation, and multiplication, root formation, and acclimatization. This study will help to grow a large number of tomato seedlings per year.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5522
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