The successful conditions of Trichoderma harzianum UzCF-55, Penicillium canescens UzCF-54, Fusarium moniliforme UzGC-12 fungal strains producing Indole-3-acetic acid (IAA) and Gibberellic acid (GA) were investigated in this work. The optimum pH of the nutrients for the strains was found to be 5.5 for Mandel's and 6.8 for the media of Czapek, with incubation temperature intervals of 28-30 ° C for 10 days. Synthesized quantitative study of the IAA by T. Harzianum UzCF-55 showed that the highest acid levels were 1.16 mg/mL and 0.74 mg/mL, respectively, on the sixth day of the exponential growth process of micromycetes, while after the ninth day they were 0.318 mg/ml and 0.17 mg/mL for the GA. During similar days of incubation, the quantities of IAA and GA synthesised by P. canescens UzCF-54 were 0.98 mg/mL and 0.38 mg/mL, both showing higher amounts than the control strain. F. The UzGC-12 moniliforme strain synthesised 0.63 mg/mL of IAA and 0.39 mg/mL of GA, respectively. High-performance liquid chromatography-mass spectrometry (HPLC-MS) studies of micromycetes showed 174.00 m/z mass fractions corresponding to IAA molecular mass, 363.00 m/z of GA7 and 361.00 m/z of GA3, suggesting strain synthesis of gibberellins.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5382
No comments:
Post a Comment