Current research focuses on the ability to manufacture solid state fermentation cellulase (CMCase, Fpase and β-glucosidase) and xylanase using Trichoderma reesei. Low lignin biomass content was preferred for fast digestibility, so we used Brown Mid Rib (BMR) 22, 24 and Non-Brown Mid Rib CSH 22 low lignin Sorghum varieties as a substrate and were subjected to two pretreatment methods (acid and alkali) to achieve maximum cellulose yield and delignification and to use maximum cellulose yield conditions for further fermentation. The highest CMCase activity (39.42 ± 4.75 IU/g), Fpase activity (13.6 ± 1.82 IU/g) and β-glucosidase activity (2.6 ± 0.19 IU/g) were obtained as substrate using pretreated BMR24 (6% NaOH 100 °C 30 min). However, the highest xylanase activity was observed at 80.9 ± 7.66 IU/g on the 7th day of incubation with pretreated (3% H2SO4 80 °C 30 min) CSH 22 Sorghum biomass as substrate, but BMR 22 and 24 mutant varieties were not active in producing xylanase. The results showed that pretreatment of sorghum biomass BMR 22, 24 and CSH22 effectively reduced lignin content and increased sugar supply during solid state fermentation.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5374
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