Tuesday, December 15, 2020

INDIGENOUS ARBUSCULAR MYCORRHIZAL FROM JOMPIE BOTANICAL GARDEN OF PAREPARE WHICH ISOLATED AT Pterospermum diversifolium | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Jompie Botanical Garden of Parepare is a regional botanical garden managed by the Center for Plant Conservation of the Botanic Gardens-Indonesian Institute of Sciences, the Ministry of Public Works and Public Housing, and the Government of the City of Parepare, South Sulawesi Province. Jompie Botanical Garden of Parepare has implemented five botanical garden functions, namely conservation, research, education, tourism, and environmental services. The master plan vegetation shows that found 90 species of plants that grow naturally and are planted by the government and the people of the city of Parepare, one of the native plants found are Pterospermuni diversivolium. In addition to various types of plants, there are also various kinds of microorganisms that have not been identified, one of which is a fungus that is capable of symbiosis with plant roots known as arbuscular mycorrhizae. The aim of the research was to determine the abundance and identify arbuscular mycorrhizal spores found in Pterospermuni diversivolium rhizosphere, using a sieve and wet techniques and staining method, which were carried out in Microbiology Laboratory, Research, and Development Center for Environment and Forestry in Makassar, Indonesia. The results showed that abundance of Glomus mycorrhizal spores, on average, 25 spores per 100 g of rhizosphere samples with morphological shapes of small spores, colored of brown to black, thin cell walls, visible hollow interior, have hyphae, smooth surface and spore diameter 136.8 - 198.5 µm. While the genus Acaulospora, has an average abundance of 21 spores per 100 g of rhizorfer samples with morphological forms of the small clear round, clear-colored, thick cell walls, surfaces appear nodules and spore diameters of 143 - 159 µm.


Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5282

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