The medicinal
properties of holy basil have made us to further concentrate on its properties.
The methanol and ethanol extracts of the plant Ocimum tenuiflorum was taken
using Soxhlet extraction method. The phytochemical profile of both the extracts
was observed for certain compounds. The extract was taken in different
concentrations and was tested against three bacterial species (Bacillus
Subtilis, Staphylococcus aureus and Escherichia coli) and three fungal species
(Aspergillus niger, Aspergillus flavus, Candida albicans). The estimation of
secondary metabolite, Alkaloid estimation was done and was observed using thin
layer chromatograph.
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